Volume 64 (2014) Issue: 2014 No#3

The investigation of DNA damage induced by adrenaline in human lymphocytes in vitro

Author(s): Radaković Milena, Đelić Ninoslav, Stevanović Jevrosima, Anđelković Marko, Kolarević Stoimir, Dačić Stefan, Stanimirović Zoran

Keywords:adrenaline, antioxidants, comet assay, human lymphocytes

Adrenaline is a neurotransmitter and hormone that plays an important role in physiological regulatory mechanisms. The objective of this study was to assess primary DNA damage in isolated human lymphocytes exposed to adrenaline using the in vitro comet assay. Dose-response of human lymphocytes was determined at concentration range of adrenaline from 0.01 µM to 300 µM for various treatment times (1h, 2h, 4h and 24h). The obtained results showed that adrenaline induced DNA damage at concentration range from 5 µM to 300 µM after 1h, 2h and 4h of treatment. The slightest DNA damage was observed after 24 h of adrenaline treatment - only the highest concentrations of adrenaline (150 μM and 300 μM) caused increased level of DNA damage. In order to evaluate the potential contribution of reactive oxygen species (ROS) in adrenaline-induced DNA damage we used antioxidants catalase (100 IU/mL and 500 IU/mL) and quercetin (100 µM and 500 µM). Co-treatment of lymphocytes with adrenaline (300 µM) and antioxidants for 1 h, significantly reduced the quantity of DNA in the comet tails. Therefore, it can be concluded that adrenaline exhibits genotoxic effects mainly through induction of reactive oxygen species and that some of the DNA damage is repaired during the first four hours following the treatment with adrenaline.