Volume 70 (2020) Issue: 2020 No#1

The performance of seven molecular methods for the detection of PRRSV

Author(s): Milićević Vesna, Kureljušić Branislav, Veljović Ljubiša, Valčić Miroslav, Stević Nataša, Savić Božidar, Radojičić Sonja

Keywords:PRRS, RT-PCR, sensitivity, Serbia

Porcine Reproductive and Respiratory Syndrome is a viral disease of swine characterized by reproductive failure of breeding animals and respiratory disorders in all categories. The first PRRS case in Serbia was recorded in 2001 after illegal import of boar semen. PRRS is economically the most important disease due to significant direct and indirect losses. Today, for routine diagnosis of PRRS in infected herds serological methods (ELISA) and molecular methods are used. Although modern diagnostic techniques are very robust, exceptional diversity of the viral strains is often the obstacle for an accurate diagnosis. To estimate the performance of seven different methods for PRRSV genome detection, twenty samples were used. However, none of the methods was able to detect all PRRSV strains. The best sensitivity was obtained by combining two methods. Until today, there is no absolutely accurate test which enables the detection of all circulating strains.